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What Role Do Autosamplers Perform in Gas Chromatography?

August 25th, 2015 6:53 am

Chromatography is a scientific process consisting of many laboratory techniques used to separate a mixture. A mixture is first dissolved in a fluid or gas and then carried to another material to create a stationary phase. This process separates the mixture by facilitating a specific speed of travel along with other factors. Chromatography may be used as a purification technique or for measuring analyte proportions in a mixture. An analyte is the actual substance being submitted to the separation process. The idea is to make one part of the mixture stationary or immobile and the other mobile to cause separation. An autosampler is often used in these lab settings to increase accuracy during the sample insertion portion of the separation process. This instrument inserts the sample into the apparatus inlets during a test for increased efficiency as well as easier reproduction. It has become the most common method for completing various types of chromatography techniques.

Analytical InstrumentsInvolved With Compound Separation Lab Techniques

Gas chromatography (GC) is one of the most common applications for sampling analytical instruments. It is used to test any compound capable of vaporizing without going through a decomposition process. GC can help laboratories test substance purity, separate specific mixtures, identify compounds, and create a pure version from a solution. Helium or a nonreactive gas acts as the carrier in the mobile phase whereas a liquid inside a column is present for the stationary phase. The gas compound reacts with the stationary phase material inside the column causing the compounds to elute at different retention times. Specially designed instruments allow scientists to compare the retention times for analytical purposes.

A chromatograph is an analytical instrument used to assist with complex sample chemical separation. It uses the column to send the sample through a gas stream at a specific rate of speed to create a desired interaction with the used column filling during the stationary phase. The stationary phase facilitates separation of the solvent compounds with each one leaving the column at varying retention times. Gas flow rate, temperature, and column length may be altered to change the separation order or retention time. A specified gas or liquid volume is inserted into the column either manually or automatically with specially designed equipment. Motion is created by molecule absorption which directly determines the rate of molecule progression. The stationary phase materials, absorption strength, and molecules types affect progression.

An autosampler is a tool used to achieve insertion into the column automatically. The insertion point is often referred to as an inlet and can be in the form of a split, split less, on-column, purge-and-trap, gas switching valve, or PTV injector. Manual insertion leaves room for error which can compromise the test results and also takes much more time. Most laboratories today use automatic injection to obtain more reliable results as well as optimize testing time. Samplers are classified based on the sample capacity, analytical purpose, or the type of technology. They are used for various separation processes including the measurement of volatile organic compounds. Automatic sampling systems have greatly improved the process of gas chromatography as well as other forms of compound separation.


A Quick Look at the Close Proctection Career

November 11th, 2014 8:24 am

Close protection officers or bodyguards have to protect their client from a large number of potential threats from assassination to the loss of confidential information. To do this they need a large number of skills, it isn’t enough just to be a large man in a suit. A bodyguard needs to be able to use evasive driving techniques, be aware of and able to put into place counter-sniper tactics, depending on the nature of the job they may need to be proficient in the use of weaponry from a Taser to an Assault Rifle.

If you want to work in the close protection environment, it is important you have good people skills. It is likely you will come across a wide variety of people when working in a close protection environment, these could range from cleaners to members of royalty. Because of this it is important that you have the skills necessary to speak to any of the people. From the moment you apply for the job, these skills will be tested. If you do not have the adequate communication skills needed then your application will be unlikely to be processed.

Another important one is common sense. You will need to have a keen eye in this line of work and keep a close watch on your surroundings and situations you find yourself in. Being proactive is key, as is confidence, both these skills are key when it comes to decision making. If you want to make sure you are credible as a close protection officer/ bodyguard then confidence is one of the key attributes you must have. Your clients will place their absolute trust in you and this comes from the confidence that you portray. Confidence in yourself will lead to confidence being shown in you by others. However, if you are not naturally confident, all is not lost. There are a number of courses that can be taken, or books read, that can help you develop this important attribute.

Always remember how important it is to be a team player as your co-workers are very important in this line of work. Without team work, all your other precious skills will be lost. Your team must always be your first priority in this line of work and comes before all else. If you are unable to demonstrate effective, selfless teamwork, this may not be the career for you. If you cannot stick close to your team and act completely selflessly then close protection may not be for.

Agelai is a forum for all Private Security Contractors, Private Military Contractors, Private Military Firms, and Close Protection Officers. If you join the community, you’ll be able to access member-only sections, and use many member-only features such as customizing your profile, sending personal messages, voting in polls and more.